Usage Method of the High-Power Objective for Optical Microscopes:

Dec 04, 2025

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Usage Method of the High-Power Objective for Optical Microscopes:

 

(1) Choose a good target: It is necessary to first center the part that needs to be further observed under low magnification, and adjust the object image to a clear level before observing under high magnification.

 

(2) Rotate the converter and replace the high-power lens. When converting the high-power lens, the rotation speed should be slow and observe from the side (to prevent the high-power lens from colliding with the glass slide). If the high-power lens touches the glass slide, it indicates that the focal length of the low-power lens has not been adjusted properly and should be operated again.

 

(3) Adjust focal length: After converting the high-power lens, observe with the left eye on the eyepiece. At this time, you can generally see an unclear object. You can move the spiral of the fine adjuster counterclockwise about 0.5-1 turn to obtain a clear object (do not use the coarse adjuster!). If the brightness of the field of view is not suitable, you can adjust the collector and aperture. If you need to replace the slide specimen, you must rotate the coarse adjuster clockwise (do not turn in the wrong direction) to lower the stage before removing the slide specimen. To make the image bigger, you need to bring the objective lens closer to the object, keep the eyepiece away from the objective lens, and vice versa.

 

Attention: When holding the mirror, it must be in the posture of holding the arm with the right hand and holding the seat with the left hand. It cannot be extracted with one hand to avoid parts falling off or colliding with other places. Handle with care and do not place the microscope on the edge of the experimental platform. It should be placed 10cm away from the edge to avoid tipping over and falling to the ground.

 

Keep the microscope clean. The optical and lighting parts can only be wiped with lens cleaning paper. Avoid blowing or wiping with a cloth, and the mechanical parts should be wiped with a cloth. Do not let water droplets, alcohol, or other drugs come into contact with the lens and stage. If contaminated, immediately wipe them off with lens cleaning paper. When placing a glass slide specimen, it should be aligned with the center of the light hole and the slide should not be placed upside down to prevent crushing the slide or damaging the objective lens. Develop the habit of simultaneously opening both eyes to observe, using the left eye to observe the field of view and the right eye to draw. Do not remove the eyepiece at will to prevent dust from falling into the objective lens, and do not disassemble various parts at will to prevent damage.

 

After use, it must be restored before being placed back in the mirror box. The steps are: remove the specimen, rotate the rotator to move the lens away from the light hole, lower the stage, lay the reflector flat, lower the collector (but do not touch the reflector), close the aperture, return the slide pusher to its original position, cover it with silk cloth and outer cover, and place it back in the laboratory cabinet. Finally, fill out the usage registration form. (Note: Reflectors should usually be placed vertically, but sometimes due to the collector not being raised to the appropriate height, the aperture may be damaged when the stage descends, so it is changed to flat placement here)

 

4 Microscope Camera

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