What are the differences in usage between oil lenses and ordinary objective lenses
The difference in usage between mirrors and ordinary objective lenses.
When using an oil lens, a drop of tar needs to be dropped on the slide, and the oil droplets cannot be detached during the rising process of the objective lens. Moreover, the magnification of the oil lens is greater than that of a regular objective lens.
When using oil mirrors, attention should be paid to:
1. After using the oil lens, first wipe off the cedar oil on the lens and specimen with a small amount of xylene on the lens wiping paper, and then wipe it clean with dry lens wiping paper. After adding tar, the field of view becomes noticeably darker. It is necessary to raise the light collector to the highest position and open the aperture to its maximum. Before using an oil lens, it is necessary to first place the target observed through a low-power lens and a high-power lens in the center of the field of view.
2. Xiangbai oil is a specialized oil for oil mirrors. Dropping a liquid with a refractive index of 1.5 under a 100x oil mirror can significantly increase the resolution of the oil mirror and improve the observation effect of the microscope. Xiangbai oil has a refractive index of 1.52, making it a superior oil for microscope oil mirrors.
3. When using a microscope oil mirror, the microscope must be upright on the table, and the mirror arm must not be bent to tilt the stage, in order to avoid the overflow of tar, affecting observation, and polluting the table.
Why use an oil mirror to achieve the highest resolution in optical microscopy
Oil mirror, one of the optical microscopes, is used when the lens is immersed in oil (usually cedar oil) to observe finer structures. It is one of the commonly used microscopes in the laboratory, with slightly higher clarity than ordinary optical microscopes, and is used to observe chlamydia, bacteria, organelles, etc
The lens of the oil lens is very small. When light passes through the air between the glass slide and the oil lens, refraction or total reflection occurs due to different media densities, reducing the amount of light entering the lens and blurring the image. If cedar oil (n=1.515) with a refractive index similar to that of the glass (n=1.52) is added between the oil lens and the glass slide, the amount of light entering the lens increases, the brightness of the field of view increases, and the image becomes bright and clear
Due to the small size of bacteria, in the study of bacterial morphology, it is often necessary to use a microscope oil mirror to observe them clearly. Therefore, it is necessary to proficiently master the use and protection methods of oil mirrors
