What are the emission wavelengths and excitation wavelengths of each band in the microscope
Ultraviolet: The excitation wavelength is 330nm-400nm, and the emission wavelength is 425nm.
Violet: The excitation wavelength is 395nm-415nm, and the emission wavelength is 455nm.
Blue: excitation wavelength: 420nm-485nm, emission wavelength: 515nm.
Green: excitation wavelength: 460nm-550nm, emission wavelength: 590nm.
Fluorescence Microscopy:
1. Fluorescence microscope has a very high ability to detect substances and has a magnifying effect;
2. Moreover, the stimulation to the cells of the detected substances is also very small, and the chromosomes of living bodies can be detected;
3. There is also the possibility of multi-step dyeing.
4. It is very good to observe the structure of fluorescein, which cannot be seen by ordinary microscopes.
6. Judging whether some substances have fluorescence, what color the fluorescence is, and whether it is the fluorescence of antibodies.
