What is fluorescence microscopy
Fluorescence microscopy is the use of ultraviolet light as a light source to irradiate the examined object, causing it to emit fluorescence, and then the shape of the object and its location are observed under the microscope. Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy, mainly because of its high sensitivity and specificity.
Fluorescence microscopy is used to study the uptake, transport, distribution and localisation of substances in cells. Some substances in cells, such as chlorophyll, can fluoresce after UV irradiation; some other substances cannot fluoresce by themselves, but if stained with fluorescent dyes or fluorescent antibodies, they can also fluoresce by UV irradiation, and fluorescence microscopy is one of the tools for qualitative and quantitative study of such substances.
Fluorescence microscopy even allows the user to determine the distribution and number of individual molecular species and their location within the cell. Studies of co-regionalisation and interactions, ion concentrations and the observation of cytosis and cytotoxicity processes between cells can be achieved. With the aid of super-resolution fluorescence microscopy, even sub-resolution structures can be imaged.
The Leica fluorescence microscope DMi8 has many innovative fluorescence features. For high-speed imaging, fast and accurate fluorescence imaging can be achieved using the external fluorescence conversion function or the automatic fluorescence illumination intensity management (FIM) system type ZL. For standard applications, fluorescence filter blocks can be easily mounted via RFID auto-ID.
