What is the difference between a fluorescence microscope and a laser confocal microscope?
There are differences in the working principles and applications between the two. It is described as follows:
fluorescence microscope
1. Fluorescence microscope is a device that uses ultraviolet light as a light source to illuminate the object being tested, causing it to emit fluorescence, and then observe the shape and position of the object under the microscope. Fluorescence microscopy is used to study the absorption, transportation, distribution, and localization of substances within cells. Some substances in cells, such as chlorophyll, can emit fluorescence after being exposed to ultraviolet radiation; Some substances themselves may not emit fluorescence, but if stained with fluorescent dyes or fluorescent antibodies, they can also emit fluorescence under ultraviolet radiation. Fluorescence microscopy is one of the tools for qualitative and quantitative research on these substances.
2. Principle of fluorescence microscope:
(A) Light source: The light source emits light of various wavelengths (from ultraviolet to infrared).
(B) Excitation filter light source: passing through a specific wavelength of light that can produce fluorescence in the specimen, while blocking
Block the useless light that stimulates fluorescence.
(C) Fluorescent specimens: generally stained with fluorescent pigments.
(D) Blocking filter: selectively transmits fluorescence by blocking excitation light that has not been absorbed by the specimen, and some wavelengths are also selectively transmitted in fluorescence. A microscope that uses ultraviolet light as a light source to emit fluorescence from the irradiated object. The electron microscope was first assembled by Knorr and Harroska in Berlin, Germany in 1931. This type of microscope uses a high-speed electron beam instead of a beam of light. Due to the much shorter wavelength of the electron flow compared to light waves, the magnification of the electron microscope can reach 800000 times, with a minimum resolution limit of 0.2 nanometers. The scanning electron microscope, which began to be used in 1963, allows people to see the tiny structures on the surface of objects.
3. Application scope: Used to enlarge images of small objects. Generally used for observation of biology, medicine, microscopic particles, etc.
