What is the difference between fluorescence microscope and inverted microscope?
The microscope is an important instrument in cell culture and related derivative experiments. At present, there are various types of microscopes on the market. It is a challenge to choose a microscope that meets your needs and is suitable. The following introduces the principles of inverted microscopes and fluorescence microscopes, so that you can choose easily.
The composition of an inverted microscope is the same as that of an ordinary microscope, and it mainly includes three parts: a mechanical part, an illumination part, and an optical part.
The composition of an inverted microscope is the same as that of an ordinary upright microscope, except that the objective lens and the illumination system are reversed, the former is under the stage and the latter is above the stage.
This structure enables the effective distance between the illumination concentrating system and the stage to be significantly expanded, which is convenient for placing thicker objects to be observed such as petri dishes and cell culture flasks (of course, glass slides are also acceptable). The working distance between them does not have to be very large.
The inverted microscope is used for the observation of microorganisms, cells, bacteria, tissue culture, suspensions, sediments, etc. in medical and health units, colleges and universities, and research institutes. It can continuously observe the process of reproduction and division of cells and bacteria in the culture medium, and can Photograph any form of this process.
It is widely used in cytology, parasitology, oncology, immunology, genetic engineering, industrial microbiology, botany and other fields.
Fluorescence microscopy is used to study the absorption, transport, distribution and localization of chemical substances in cells.
For the object to be inspected, there are two ways of generating fluorescence: autofluorescence, which emits fluorescence directly after ultraviolet irradiation; secondary fluorescence, which can emit fluorescence after the object to be observed is treated with fluorescent dyes and then irradiated with ultraviolet light.
Some substances in cells, such as chlorophyll, produce autofluorescence after being irradiated by ultraviolet rays; some substances cannot fluoresce by themselves, but if they are stained with fluorescent dyes or fluorescent antibodies, they can also emit secondary fluorescence after being irradiated by ultraviolet rays.
The fluorescence microscope uses a point light source with high luminous efficiency, and emits light of a certain wavelength (ultraviolet light 365nm or violet blue light 420nm) through the color filter system as the excitation light, after exciting the fluorescent substances in the specimen to emit various colors of fluorescence, and then Observe by magnification of the objective and eyepieces.
Under the strong contrast background, even if the fluorescence is very weak, it is easy to identify and has high sensitivity. It is mainly used for the study of cell structure and function and chemical composition.
