What is the difference between inverted fluorescence microscopy and laser confocal microscopy
Laser confocal microscope is a set of observation, analysis, and output systems that use laser as the light source, conjugate focusing principle and device on the basis of traditional optical microscope, and digital image processing of the observed object using a computer. The main systems include laser light sources, automatic microscopes, scanning modules (including confocal optical path channels and pinholes, scanning mirrors, detectors), digital signal processors, computers, and image output devices (displays, color printers). By using a laser scanning confocal microscope, it is possible to perform tomography and imaging of the observed sample. Therefore, it is possible to observe and analyze the three-dimensional spatial structure of cells without damage.
At the same time, laser scanning confocal microscopy is also a powerful tool for dynamic observation of live cells, multiple immunofluorescence labeling, and ion fluorescence labeling. It accurately analyzes the essence of the spectrum and distinguishes signals from different labels with highly overlapping emission spectra.
The most important thing is that for multi-color fluorescence staining, it can completely eliminate the influence of fluorescence crosstalk, while minimizing the loss of sample fluorescence signal. These are all things that ordinary mirrors cannot achieve.
Three differences
1. Fluorescence microscope: Fluorescence microscope is a fundamental tool in immunofluorescence cytochemistry. It is composed of main components such as light source, filter plate system, and optical system. It is to use a certain wavelength of light to excite the specimen and emit fluorescence, and to observe the fluorescence image of the specimen through an objective and eyepiece system amplification.
2. Laser confocal microscopy: Laser scanning confocal microscopy technology has been used in the study of cell morphology localization, three-dimensional structural recombination, dynamic change processes, and provides practical research methods such as quantitative fluorescence measurement and quantitative image analysis. Combined with other related biotechnology, it has been widely applied in molecular cell biology fields such as morphology, physiology, immunology, genetics, etc.
