What is the difference between using oil lenses and ordinary lenses?
1. Different usage methods
Ordinary objective lenses do not need to add any medium and can be used directly to observe objects. The working distance of oil lenses is much shorter than that of high-power dry-system objective lenses. The shortest one is only 0.1mm, and the focusing procedure is different from that of dry-system objective lenses. It must be operated during operation. Be particularly careful to prevent the oil lens from crushing the specimen or damaging the oil lens. When using an oil lens for the first time, you can also adjust the focus according to the focusing procedure for dry-type objective lenses. That is, first lower the lens barrel until the objective lens is closest to the cover glass, but never press it on the specimen, and then raise the objective lens to adjust the focus. However, this method has the disadvantage of squeezing out the immersion liquid and causing cavitation. If when the coarse focus screw is raised, the lens barrel has risen to the point where the oil immersion lens is away from the oil droplets, and the object being inspected still cannot be found, it must be adjusted again.
The lens oil used for oil lenses is cedar oil or paraffin oil. Due to the high viscosity of cedar oil, it is troublesome to wipe it off after use, so many people use paraffin oil instead. When dripping the immersion solution, try to avoid the formation of bubbles. If bubbles are formed, use the tip of a dissecting needle to push the bubbles aside to avoid affecting the observation. After using the oil lens, lift the lens barrel about 2cm, turn the oil lens away from the optical axis, first wipe off most of the oil on the lens with lens cleaning paper, and then wipe off the remaining oil on the lens with lens cleaning paper dipped in a small amount of xylene Finally, wipe off the xylene on the lens with dry-wiping lens paper.
2. Different clarity
The oil lens is used to observe finer structures. It is one of the microscopes commonly used in laboratories. The clarity is slightly higher than that of ordinary optical microscopes. It is used to observe chlamydia, bacteria, cell organelles, etc.
