What is the optical system of fluorescence microscope?
Under the microscope, due to the optical characteristics of certain substances, ordinary upright microscopes cannot see their internal structures clearly, but they have another characteristic. For example, some substances in cells, such as chlorophyll, can fluoresce after being irradiated by ultraviolet rays; although some substances themselves cannot fluoresce, they can also fluoresce after being dyed with fluorescent dyes or fluorescent antibodies after being irradiated with ultraviolet light. Using the optical characteristics of this substance, a professional microdisplay device, namely a fluorescence microscope, has been developed. Fluorescence microscopy is a tool for qualitative and quantitative research on such substances. The precise definition of a fluorescence microscope refers to a microscope that selects a certain wavelength of excitation light emitted by a high-pressure mercury lamp or a similar light source to excite certain substances labeled with fluorescent dyes in cells to emit fluorescence, and to observe the distribution of a specific component of the cell. Semi-quantitative assays can also be performed. Fluorescence microscopy is one of the tools for qualitative and quantitative research on such substances.
The adjustment of the microscope illumination optical path system is to make the field of view of the microscope receive uniform and sufficient illumination. When the microscope is first installed and debugged, the illumination optical path system must be adjusted well. This is an important means and the most basic requirement to use the microscope correctly and obtain correct and reliable results. In addition, correctly mastering the adjustment of the illumination optical path system is a necessary step after replacing the light source bulb during the use of the microscope, and it is also a necessary means to check the performance of the microscope from time to time during daily use. Fluorescence microscopes differ from ordinary microscopes in the following ways:
1. The illumination method is usually episcopic, that is, the light source is projected on the sample through the objective lens;
2. The light source is ultraviolet light, the wavelength is shorter, and the resolution is higher than that of ordinary microscopes;
3. There are two special filters, the one in front of the light source is used to filter out visible light, and the one between the eyepiece and objective lens is used to filter out ultraviolet rays to protect the human eye.
Fluorescence microscope is also a kind of optical microscope, the main difference is that the excitation wavelength of the two is different. This determines the difference between the fluorescence microscope and the ordinary optical microscope in terms of structure and usage.
Fluorescence microscopy is an essential tool in immunofluorescent cytochemistry. It is composed of main components such as light source, filter plate system and optical system. It is to use a certain wavelength of light to excite the specimen to emit fluorescence, and to observe the fluorescence image of the specimen by amplifying the objective lens and eyepiece system. It is widely used in the fields of biology and medicine research.
