Which is better, a light microscope or an electron microscope?
With the rapid development of science and technology, the application of electron microscope in the field of microscopy is very broad prospects, compared with the optical microscope shows its unique superiority. However, because of the application of optical microscope and electron microscope technology and fields are different, electron microscope and can not completely replace the optical microscope.
Application in biology, the resolution of optical microscope is far less than the electronic microscope, because the resolution of optical microscope by the diffraction limit, so its resolution can not be less than half of the wavelength of the incident light. That is to say, if the incident light of 400nm, then the observation object can not be less than 200nm, but because it can be real-time, dynamic observation, the status in biology is incomparable, just like in the field of biology can not leave the fluorescence microscope and confocal and other optical microscopes. The electron microscope due to the use of electron beam to scanning imaging, its resolution can easily reach the nanometer level, which is irreplaceable for the application of high-resolution imaging.
In metallographic applications, the magnification of the electron microscope is also far more than the optical microscope, the maximum magnification of the modern electron microscope has been more than 3 million times, while the maximum magnification of the optical microscope is about 2,000 times, so the electron microscope is able to directly observe the atoms of certain heavy metals and crystals in the atom of a neatly arranged array of dots.
What is the maximum magnification of an optical microscope?
Optical microscope magnification, mainly by the objective lens on the object to do the actual magnification, the eyepiece on the objective lens magnification of the image of the secondary magnification. The objective lens of an optical microscope can only go up to 100 times, and the eyepiece has a 10x
16x
The maximum has 20 times the eyepiece. So the objective lens 100 times, eyepiece 20 times the case, the total magnification up to 2000 times. But this 2000 times is only the number of 2000 times, not the true meaning of the magnification of 2000 times. For example, when the eyepiece is used 10 times, the objective lens 100 times, the total magnification of 1000 times, because the actual magnification of the objective lens is still 100 times, only the eyepiece from 10 times to 20 times. It looks like 2000 times
1000 times, the difference is twice as much, but in reality it is only a difference of 10 times.
