Why can't electron microscope replace optical microscope?

Sep 13, 2023

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Why can't electron microscope replace optical microscope?

 

The electron microscope is based on the principle of electron optics. Electron beams and electron lenses are used instead of light beams and optical lenses, so that the fine structure of matter can be imaged at a very high magnification. Although its resolving power is far better than that of optical microscope, it is difficult for electron microscope to observe living organisms because it needs to work in vacuum, and the irradiation of electron beam will also damage biological samples, so it can't completely replace optical microscope, and their cost and suitable working range are different. I hope my answer can help you.


The reasons why electron microscope can't completely replace optical microscope are as follows:
1. The electron microscope belongs to the optical microscope, and accessories such as CCD, display screen or computer are added. This can only be said to be a video microscope. In the whole imaging process, CCD replaces the human eye. Because in video imaging, electronic amplification belongs to virtual amplification, and in terms of pixels, photosensitive effect and other factors, it is too different from human eyes, so it is too different from visual microscope effect;


2. There is also the most important reason. CCD belongs to plane imaging, but human eyes, especially binocular observation, will have a strong stereoscopic effect, which is the reason why the contrast depth of field between them is too large;


3. Electron microscopes are mostly expressed as scanning electron microscopes, which are much better than ordinary optical microscopes, but they are rarely used in industry because of their high price.

 

2 Electronic microscope

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