Why electron microscopy is not a replacement for light microscopy
The electron microscope uses the principle of electron optics, replacing the light beam and optical lens with an electron beam and an electron lens, so that the fine structure of the material is imaged under very high magnification. It has been far better than the optical microscope, but the electron microscope needs to work in vacuum conditions, so it is difficult to observe living organisms, and the irradiation of the electron beam will also make the biological samples irradiated damage, so it can not completely replace the optical microscope, and their cost is not the same, and suitable for a different range of work. I hope my answer can help you.
The reasons why electron microscope can not completely replace optical microscope are as follows:
1. electronic microscope belongs to the optical microscope based on the addition of CCD, display or computer accessories, which can only be said to be a video microscope, the entire imaging process, CCD instead of the human eye. Because the video imaging, electronic magnification is false amplification, and on the pixel, light-sensitive effect and other factors, with the human eye is too big a difference, so with the visual microscope effect is too big a difference;
2. There is a most important reason, CCD belongs to the plane imaging, and the human eye, especially binocular observation, will produce a strong sense of three-dimensional, which is the two contrast depth of field effect is too large;
3. Electron microscope is mostly expressed as electron scanning microscope, this microscope effect will be much better than the general optical microscope effect, but because of the high price, so few applications and industry.
