Why is the imaging quality of laser confocal microscopy better?
The principle of laser confocal microscopy is that the light beam emitted by an LED light source passes through a multi hole disk and objective lens, and is focused on the surface of the sample. Afterwards, the beam is reflected back to the measurement system through the surface of the sample. When passing through the pinholes on the MPD again, the reflected light will only retain the focused light points. Finally, the beam of light is reflected by a splitter and imaged on the camera.
Why is the imaging quality of laser confocal microscopy better?
1. The laser confocal microscope uses laser scanning technology. Compared with the broad-spectrum light source of traditional microscopes, laser scanning technology can accurately locate and focus on specific areas of the sample, thereby improving the resolution and accuracy of imaging. Meanwhile, laser scanning technology can eliminate scattering and background signals in the sample, thereby improving the contrast of imaging. Meanwhile, the monochromaticity of the laser makes the imaging clearer.
2. Laser confocal microscopy has a larger optical aperture (the ability of the microscope to receive light emitted by the sample) and a high numerical aperture objective (the magnification of the lens), making imaging clearer and more detailed.
3. The laser confocal microscope uses a highly sensitive photomultiplier tube as the detection element, which can exhibit high sensitivity to weak fluorescence signals. It can also eliminate background noise by reducing the excitation range and using optical slicing** Equipped with highly sensitive photodiode detectors, the laser confocal microscope can quickly and accurately detect light signals and convert them into electrical signals.
Unlike traditional optical observation, photodiode detectors can detect individual photons, making imaging more sensitive and accurate. This high sensitivity detector can also obtain clear images under low light intensity conditions.
The VT6000 laser confocal microscope is based on the conjugate confocal principle of a pinhole point light source, with nanometer level longitudinal resolution. Coupled with a high-speed scanning module, professional analysis software has multi area and automatic measurement functions, which can achieve rapid and automated measurement. It also provides a series of contour size parameters such as height, width, and angle to characterize surface quality.
