A wide variety of fluorescence microscopy imaging techniques
In order to meet different fluorescence imaging needs, in addition to fluorescence microscopes, various fluorescence microscopy imaging solutions have been developed:
Wide-field high-definition imaging systems, such as Leica THUNDER Imager, use Leica’s innovative Clearing technology to efficiently remove non-focal plane interference signals during imaging, present clear images, and have the advantages of high-speed imaging;
Confocal laser scanning microscope, using pinholes to eliminate non-focal plane interference, realize optical sectioning, and obtain high-definition images and three-dimensional images;
Ultra-high-resolution microscopes and nano-microscopes that break through the diffraction limit, and can observe fine structures smaller than 200nm;
A multiphoton imaging system that uses the principle of multiphoton excitation to perform deep imaging of thick tissues and living bodies;
Light-sheet imaging technology with high spatio-temporal resolution, fast imaging speed, high resolution, low phototoxicity, especially suitable for development, living dynamic observation and other research;
Fluorescence lifetime imaging (FLIM), which is not affected by factors such as fluorescent substance concentration, photobleaching, and excitation light intensity, can perform more in-depth and accurate functional measurements;
Fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS), measure the molecular number and diffusion coefficient of fluorescent molecules, so as to analyze molecular concentration, molecular size, viscosity, molecular motion, molecular binding/dissociation, optical properties of molecules, etc.;
Total internal reflection fluorescence microscopy (TIRF), with extremely high z-axis resolution, is very suitable for the study of molecular structure and dynamics on the surface of cell membranes.
Fluorescence microscopy imaging technology is widely used and rich in types, and new technologies are constantly emerging. You can choose the appropriate technology to complete your own research.
