Advantages of laser confocal microscopy
Laser confocal microscope is a new technology that combines laser technology, microscope technology, **fluorescence technology, computer and image processing technology, precision mechanical technology, etc., high, precise and sharp cell analysis and engineering technology in one. It has become a new generation of powerful research tools in the fields of morphology, molecular cell biology, neuroscience, pharmacology, genetics and so on.
Laser confocal microscope is nowadays * the most advanced optical microscope, its main advantages are:
1, laser as a light source, in the corresponding fluorescent probe labelling, point by point scanning of the sample, layer by layer to obtain two-dimensional optical cross-sectional images, with the function of "cell CT", and through the computer three-dimensional reconstruction software support, to obtain three-dimensional images, and can be rotated at an arbitrary angle, observation of the cells, tissues, three-dimensional morphology and spatial relationships. It can be rotated at any angle to observe the three-dimensional shape and spatial relationship of cells and tissues;
2,It can observe the living cells and tissues without damage, and dynamically measure the intracellular Ca ion concentration and pH value and other physiological information of the living cells;
3,It can be used as a "light knife" to complete intracellular "surgery" on cell membrane fluidity, intercellular communication, cell fusion, cytoskeleton elasticity measurement. This technology can carry out in situ dynamic quantitative observation and measurement of living cells and tissues.
