Analysis of Microscope Optical Failures
The dual images of the microscope do not overlap. In binocular observation, sometimes there may be a phenomenon of non coincidence of the two images. The occurrence of non coincidence of the two images does not matter whether it is due to inconsistent compensation of the lengths of the two barrels The significant difference in magnification between the two eyepieces is due to three reasons: the movement of the binocular prism caused by vibrations during use or transportation. For the first two reasons, they are calibrated and selected at the factory, and can be solved by paying attention to the methods and matching during use.
The third reason for microscopes is quite common. At this time, the binocular housing should be opened, a cross ruler should be placed on the platform, and 10X dividing eyepieces should be inserted into the left and right barrels for observation. The position and angle of the binocular prisms should be corrected, and when the binocular eyepieces are rotated to different angles for observation, the position of the cross ruler should be at the same position in the field of view of the left and right eyepieces, and then fixed firmly.
When observing with a binocular microscope, sometimes there may be inconsistencies in brightness and color between the left and right fields of view, which can affect the observation. This is due to the damaged spectral film of the prism. In this case, the prism should be removed and sent to the microscope manufacturer for re coating before assembly and use.
Characteristics and Applications of Microscope Dark Field Illumination
Microscope dark field illumination improves the actual resolution and contrast of the microscope
The dark field adopts oblique light illumination, fully utilizing the aperture angle of the objective lens, and the contrast of the dark matrix is good, which improves the actual resolution.
For example, when observing a sample containing pearlite in a bright field, there are many layers of pearlite that are difficult for the objective lens to distinguish due to their fineness. When converted to dark field illumination, the layered images in the same area are clearly visible, indicating that the actual resolution of the objective lens has been improved in the dark field.
In addition, there are many ultrafine particles in steel that cannot be identified in bright fields, and some can be seen as faint dots. But if Olympus microscope is used for dark field illumination, by eliminating the bright background caused by the scattered light of these particles, the contrast of the diffraction image of these particles is enhanced. Many small bright spots can be seen distributed on the dark matrix, and some even present various colors, making the small dots clear and distinguishable. Just like seeing stars at night, although we cannot distinguish the details of these particles, we can detect their presence.
