Basic Microscope and Digital Camera Operation Procedure
1. Fluorescent light source warm-up. (for fluorescent microscopes)
2. with no slide in place, white balance by adjusting the shooting lever to the digital camera.
3. Adjust the shooting lever to the eyepiece viewing position.
4. Adjust the pupil distance
5. Adjust the parallax
6. Find the target under the bright field, low magnification objective lens, and focus it.
7. Change the appropriate objective lens and focus.
8. Adjust the brightness of the light source
9. adjust the spotting lens
10. Adjust the filter or aperture as necessary.
When a clear image is visible in the eyepiece, adjust the microscope to the digital camera as follows:
11. Adjust the shooting lever to transfer the image to the digital camera.
12. In bright field, check if the image is in focus, if not, turn the fine focus knob to focus. This should be a bright field of view and keep the camera in high frame rate. 13.
13. Adjust the colour of the camera, either by doing manual white balance or manually adjusting the gain values for red, green and blue as required.
14. Take photos.
When shooting, bright field of view situation, cmos camera should be gain value lower, and adjust the saturation as needed.
For fluorescent photos, select the appropriate gain value according to the recommendation, and adjust the camera frame rate down to ensure that the photos are taken with low noise.
If you need to take better fluorescence pictures, the camera should be optimised for micrographs and be able to perform cooling to reduce noise. It is also good to have an adjustable frame rate to reduce noise.
