Microscope commissioning and use
After understanding the name, structure and function of the main components of the microscope, in order to better play a variety of functions of the microscope, improve work efficiency, to ensure that in the process of micro-observation and micro-photography to achieve the best results, the use of personnel must understand and master the correct debugging method of the microscope and the use of methods. Especially in the new generation of microscopes, with a variety of functions, can carry out a variety of microscope inspection methods of observation, the correct method of trial adjustment and the use of methods is particularly important. The following is an example of universal research microscope, briefly describing the debugging and use methods.
1. Adjustment of microscope illumination light path system
In order to make the field of view of the microscope can be uniform and sufficient illumination, in the initial installation and commissioning of the microscope, it is necessary to adjust the illumination optical system, which is the correct use of the microscope, and to obtain the correct and * results of the important means and * basic requirements. In addition, the correct mastery of the adjustment of the illumination light path system is the use of the microscope in the process of replacing the light source bulb after the necessary steps, but also in the daily use of the process from time to time to test the performance of the microscope necessary means. The adjustment of microscope illumination light path system has the following four main contents:
(1) Preliminary adjustment of the illumination light source chamber outside the microscope
① Firstly, open the shell of the lamp chamber, press the spring clip to load the halogen bulb into the socket, avoid direct finger contact with the bulb when installing (can be separated by soft cloth or paper), so as not to leave fingerprints and other dirt on the bulb, which will affect the service life of the bulb.
② the lamp room on the desktop, turn on the power, with a special screwdriver to adjust the lamp focusing knob hole (marked '← →'), so that the filament projection on the wall 1-2m away from the filament imaging adjusted to a clear; and then adjust the lamp's high and low position of the adjustment of the filament holes (marked '- - -'), so that the filament of the high and low position of the lamp (marked '- - '), so that the lamp is not in contact with the light, so as to avoid direct finger contact with the bulb (available soft cloth or paper insulation). '), so that the filament position is appropriate; and then adjust the light of the left and right position of the adjusting screw holes (marked with "──"),so that the filament position is appropriate.
(2) light source emitter (filament) in the microscope position of the test and correction The purpose is to light emitter of the image of the end squarely into the field of view of the objective lens, from the perspective of the light source to ensure that the field of view of the microscope by the full and uniform illumination, which is a prerequisite for adjusting the Kuhler illumination system. Basic tools needed: the centring telescope is already equipped when the microscope is purchased.
① Unplug the hairy glass sleeve in the lamp housing and put the lamp chamber back on the microscope; ② Select a 10× objective lens.
② selected 10 × objective lens, open bright light source procedures to find the sample and focus clear, and then change to 40 × objective lens to focus the sample clear (40 × objective lens can see the full picture of the filament); ③ the aperture of the spotting scope and the microscope.
③ Open the aperture diaphragm and field of view diaphragm of the spotting scope to * large; ④ Unplug one of the eyepieces.
④ Unplug one of the eyepieces and replace it with a centred telescope, grasp the white part and stretch the black eyepiece with the other hand, then you can see the filament image in the field of view.
⑤ such as filament position is not appropriate, adjust the '─' hole, the filament image along the horizontal direction, adjust the '─' hole, the filament image along the vertical direction, until the filament image is adjusted to just fill the aperture of the objective lens of the light round image; ⑥ after the adjustment, the hair of the eyepiece, the hair of the eyepiece, the hair of the eyepiece, the hair of the eyepiece, the hair of the eyepiece.
⑥ adjustment is complete, the hair glass sleeve back to the original position, pull out the focusing telescope, back to the eyepiece for the next step in the adjustment. The adjustment of the illumination light source room outside the microscope and the calibration of the position of the light source luminous body in the microscope mentioned above should only be carried out when the microscope is first installed and debugged and the bulb is replaced, and it should not be adjusted at random when the microscope is used in normal times. In case of disorder, the microscope can be adjusted back to its original state according to the above steps.
