Carbon dioxide detector analyzes the cause of low carbon dioxide detected by enzyme method
The reagents stored in airtight storage and the reagents stored in the open store increased with the prolongation of storage time. This phenomenon was not mentioned in the kit manual; the reagent blank absorbances of the two storage methods were matched within 12 hours. The t test showed no significant difference. In 12h to 72h, the blank absorbance of the two reagents all decreased continuously, but the decreasing speed was slower. The reagent blank absorbance of airtight storage is slower than that of open storage; at this stage, the reagent blank absorbance values of the two storage methods are significantly different by paired l test. For the detection of carbon dioxide, a carbon dioxide detector can be used for detection and analysis, which provides convenience for related research.
It can be seen that the blank absorbance value of the reagent has experienced a process of rising first and then falling, but the reagent manual did not mention it. Theoretically, the reaction of carbon dioxide in the air with the reagents contained in the open bottle will only cause the reduction of N and DH, so that the absorbance of the reagent blank will continue to decrease after redissolving; but the absorbance of the reagent blank will increase within 121 μm after the reagent is redissolved. It is indeed puzzling.
In daily work, we generally refer to the instructions of the reagents. After the reagents are reconstituted and placed at room temperature for about 10 nlln, the reagents are poured into the uncapped special bottles for the machine, and they are only calibrated once a day before taking samples. The carbon dioxide concentration of the samples is calculated by the formula [C sample-(blank absorbance-sample absorbance) and [blank absorbance-standard absorbance) and standard concentration] we can see that after calibration, the default blank absorbance and (blank absorbance-standard absorbance) of the instrument remain unchanged, while in fact Within 12 hours after the reconstitution of the reagent, the blank absorbance of the reagent has been increasing with time, so the carbon dioxide concentration of the sample is measured after the instrument is calibrated for a period of time, and the measured result must be low, and the low degree increases with the increase of the blank absorbance of the reagent.
Carbon dioxide detector Aiming at the phenomenon that the reagent blank absorbance of the reconstituted reagent rises continuously within 12 hours, the author did the test of 1.2.2 above. This is based on room temperature conditions. Whether the serum is airtightly stored within 4 hours, the difference in the results is not significant. The two-point endpoint method is better than the one-point endpoint method. This is caused by the different calculation formulas of the absorbance of the two detection methods. Obviously, the AI change is greater than /l2. Also according to the sample carbon dioxide concentration calculation formula, the one-point endpoint method can be obtained. The measured results are reduced by more than Two-point endpoint method.
