Catalogue of 7 Common Methods for Observing Microscopes
1) Let's take a familiar method of microscopic examination - bright field microscopy, which can be performed by all microscopes;
2) A phase contrast microscope uses the difference in optical path of the object being examined for microscopic examination, which effectively utilizes the interference phenomenon of light to transform the phase difference that cannot be distinguished by the human eye into a distinguishable amplitude difference. Even colorless and transparent substances can become clear and visible;
3) Differential interferometry is the use of a specially designed Wollaston prism to decompose a beam of light. The vibration directions of the split beams are perpendicular to each other and of equal intensity. The beams pass through the object at two points that are very close together, with slight differences in phase. Due to the extremely small gap between the two beams and the absence of ghosting, the image presents a three-dimensional feeling;
4) The dark field of vision is actually a dark field illumination. Its characteristics are different from the bright field of vision, as it does not directly observe the illumination light, but instead observes the reflected or diffracted light of the object being tested. Therefore, the field of view becomes a dark background, while the object being inspected presents a bright image. The special accessory required for observing a dark field of view is a dark field spotlight;
5) Polarization microscope is a type of microscope used to identify the optical properties of fine structures of substances. Any substance with birefringence can be clearly distinguished under a polarizing microscope. Of course, these substances can also be observed by staining, but some are impossible and must be observed using a polarizing microscope;
6) In 1975, Dr. Robert Hoffman invented it. In 2002, ZL expired, and various microscope manufacturers introduced RC technology products named after themselves. The principle of oblique light irradiation on the specimen produces refraction and diffraction, and the light passes through the objective lens density gradient adjuster to produce different shadows, resulting in differences in brightness on the surface of the transparent specimen and increasing observation contrast
7) Fluorescence microscopy is a technique that uses short wavelength light to irradiate an object that has been stained with fluorescein, excite it, and produce fluorescence of a growing wavelength, and then observe it.
