Common Malfunctions, Causes, and Troubleshooting in Microscope UseCommon Malfunctions, Causes, and Troubleshooting in Microscope Use

Jul 04, 2025

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Common Malfunctions, Causes, and Troubleshooting in Microscope Use

 

1. The microscope is cloudy and unclear
Main reason: Unclean or moldy lenses.
Exclusion method: When there is dust or dirt on the lens, use a brush or feather to remove it, then use lens paper or a degreasing cotton dipped in a small amount of anhydrous alcohol or ether to carefully wipe along a circular trajectory, but do not let the wiping liquid flow away.


2. The edge of the indentation cannot be clearly seen inside the mirror
Main reason: Some lenses are loose.
Exclusion method: Re tighten the loose parts of the lens.


3. The scale value of the reading microscope does not coincide with the scale of the standard ruler
Main reasons: Loose objective lens or missing washer at the connection between objective lens and barrel, resulting in changes in focal length.
Exclusion method: Tighten the objective lens. If the washer is missing, its thickness should be repeatedly adjusted and a suitable washer should be installed until the position with the smallest scale error.


4. The scale value of the reading microscope is larger than that of the standard ruler
Main reason: The tube has grown, possibly due to loose tube joints.
Exclusion method: Re tighten the tube joint.


The relationship between the focal length of microscope objective and eyepiece
1. The eyepiece and objective are both convex lenses with different focal lengths.


2. The focal length of the convex lens of the objective is smaller than that of the convex lens of the eyepiece. The objective lens is equivalent to the lens of a projector, and objects are inverted and magnified into a real image through the objective lens. The eyepiece is equivalent to a regular magnifying glass, and the real image is then transformed into an upright and magnified virtual image through the eyepiece. Objects that reach the human eye through a microscope become inverted magnified virtual images.


3. The relationship between the length of the eyepiece and objective lens and the magnification is opposite. The longer the eyepiece lens, the smaller the magnification; The longer the objective lens, the greater the magnification.


4. The overall magnification of a microscope is equal to the magnification of the eyepiece multiplied by the magnification of the objective lens.

 

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