Differences between confocal laser scanning microscope and scanning electron microscope
Laser confocal microscope and scanning electron microscope both use point source to scan imaging point by point, and adjust the magnification by controlling the scanning drive range. Laser confocal microscope works through laser scanning and can obtain three-dimensional images. Scanning electron microscopy modulates imaging using various physical signals excited by a fine focused electron beam scanning the surface of a sample, and can only obtain two-dimensional images, not three-dimensional images.
1. Different extreme resolutions (amplified signal sources vary)
Laser confocal: maximum resolution of 150nm
Scanning electron microscope: 20nm~0.8nm
2. Different scanning driving methods
Laser confocal: laser mirror controls laser scanning range and scanning speed
Scanning electron microscope: Electromagnetic coil controls the scanning range and speed of the electron beam
3. Different stereoscopic imaging
Laser confocal: The sample is driven by a nano precision stepper motor to image layer by layer in the Z-axis direction, and the software synthesizes the set images of each layer into a clear three-dimensional image
Scanning electron microscopy: Single frame images have a large depth of field and belong to two-dimensional images
4. Different application scope
Laser confocal: several times to several thousand times
Scanning electron microscope: several times to several hundred thousand times
5. Different working environments
Laser confocal: can test samples in atmospheric environment
Scanning Electron Microscope: Testing Samples in High Vacuum Environment
