How to adjust the center of the mercury lamp of a fluorescence microscope?
In order to adjust the center of the fluorescent mercury lamp, we must first understand the basic structure of the fluorescent microscope light box.
1Light box structure
Lamphouse Frame: A mechanical bracket structure that fixes the internal structure of the fluorescent light box and has good heat dissipation effect.
Mercury Arc Lamp: A high-pressure short-arc mercury lamp that produces a continuous spectrum lamp to provide illumination for fluorescence experiments.
Reflector (Mirror): A mirror structure similar to a bowl with the mouth facing the mercury lamp. Because the light emitted by the mercury lamp bulb is scattered in four directions, in order to ensure better lighting efficiency, this light collection device is added to collect the light emitted by the mercury lamp in the opposite direction to the lighting direction.
Collector lens: The purpose is to collect the light obtained by the mercury lamp and the reflector to form a highly efficient beam of parallel light, which is provided to the fluorescent lighting system.
Now that we understand the structure of the light source, let's explain how to adjust the mercury lamp
2How to adjust the mercury lamp
1) If there is an adjustment attachment, use the adjustment attachment. If there is no adjustment attachment, you can put a flat piece of white paper on the sample holder to focus 3?C[Kq
2) Adjust the collector focus knob to clearly adjust the original image and the mirror image (picture below). At this time, you will find that there are two images, but their positions are one up and one down or one left and one right.
3) Adjust the mercury lamp position adjustment knob to adjust the two images to coincide with each other.
4) At this time, the center has been adjusted and the sample can be taken for observation. If the brightness in the field of view is uneven, you can fine-tune the focus knob.
Knowing the above points, you can solve some common microscope failures.
