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How to use bacterial biomicroscope

Dec 05, 2023

How to use bacterial biomicroscope

 

How to use biological microscope


Taking the mirror and placing it
1. Hold the mirror arm with your right hand and support the mirror base with your left hand.


2. Place the microscope on the experimental table, slightly to the left (the microscope is placed about 7 cm from the edge of the experimental table). Install the eyepiece and objective lens.


to light
3. Rotate the converter to align the low-magnification objective lens with the clear aperture (the front end of the objective lens should be kept 2 cm away from the stage).


4. Aim a larger aperture at the clear aperture. Keep your left eye looking into the eyepiece (open your right eye to facilitate drawing at the same time later). Rotate the reflector so that the light is reflected into the lens barrel through the light hole. Through the eyepiece, you can see a bright white field of view.


observe
5. Place the slide specimen to be observed (it can also be made from a piece of thin paper with the word "6" printed on it) on the stage and press it with a pressing clamp. The specimen should be facing the center of the light hole.


6. Turn the coarse focusing screw to slowly lower the lens barrel until the objective lens is close to the glass specimen (look at the objective lens to prevent the objective lens from touching the glass specimen).


7. Look into the eyepiece with your left eye, and at the same time, turn the coarse focus screw in the opposite direction to slowly raise the lens barrel until you can see the object image clearly. Then turn the fine focus screw slightly to make the object image you see clearer.


8. Use of high-magnification objective lenses: Before using high-magnification objective lenses, you must first find the object to be observed with a low-magnification objective lens and adjust it to the center of the field of view, then turn the converter and change to the high-magnification lens. After switching to a high-magnification lens, the brightness in the field of view becomes darker, so generally choose a larger aperture and use the concave surface of the reflector, and then adjust the fine focus screw. The number of objects viewed becomes smaller, but their size increases.


tidy
8. After the experiment, wipe the surface of the microscope clean. Turn the converter, tilt the two objective lenses to both sides, slowly lower the lens barrel to the lowest position, and place the reflector vertically. Finally, put the microscope into the mirror box and return it to its original place.


"Precautions":
1. It is strictly forbidden to pick up the microscope with one hand.


2. If you need to move the microscope, be sure to lift the microscope and put it in the appropriate position. Do not push the microscope (the vibration caused when pushing may cause the internal parts of the microscope to loosen, remember!!). Please be careful when using the microscope.


3. When using a microscope, the height of the seat should be appropriate. When observing, you should be accustomed to observing with both eyes at the same time. The aperture and light source brightness should be appropriate. Otherwise, you will easily feel tired during long-term observation.


4. When rotating the rotating disk, be sure to lower the stage to the lowest point to avoid scratching the eyepiece lens due to improper operation.


5. When staining specimens or performing any other operations, the slides should be removed and placed back on the stage for observation after the operation is completed. Do not operate on the stage to prevent dye or other liquids from flowing into the microscope or damaging the lens.


6. After observing one material, when you want to change another material, be sure to lower the stage to the lowest point, change the glass, and then refocus according to the standard procedure. Do not change the specimen directly to avoid scratching the lens or Slide specimen.


7. After using the microscope, lower the stage to the lowest point, align the low-magnification lens with the round hole in the center of the stage, roll up the power cord, cover it with a dust cover, and store it in a storage cabinet.

 

4 Microscope

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