1. The microscope should be placed on the table slightly to the left during the experiment, and the mirror base should be about 6 to 7 cm away from the edge of the table.
2. Turn on the light source switch and adjust the light intensity to an appropriate size.
3. Rotate the objective lens converter so that the low magnification lens is facing the light hole on the stage. First adjust the lens to about 1 ~ 2cm from the stage, then look into the eyepiece with your left eye, then adjust the height of the condenser, and adjust the aperture diaphragm to the maximum, so that the light is injected into the lens barrel through the condenser In this case, the field of vision is bright.
4. Put the glass slide to be observed on the stage, so that the observed part of the glass slide is located in the center of the light hole, and then clamp the slide glass with the specimen clip.
5. First observe with a low magnification lens (objective 10X, eyepiece 10X). Before observation, turn the coarse focus handwheel to raise the stage, and the objective lens gradually approaches the glass slide. Care should be taken not to let the objective lens touch the slide, in order to prevent the lens from crushing the slide. Then, look into the eyepiece with the left eye, and don't close the right eye at the same time (to develop the habit of observing with a microscope with both eyes open, so that the right eye can look at the drawing while observing), and turn the coarse focus handwheel to make The stage is lowered slowly, and a magnified image of the material in the slide can be seen for a long time.
6. If the object image seen in the field of view does not meet the experimental requirements (the object image deviates from the field of view), you can slowly adjust the moving handle of the stage. When adjusting, it should be noted that the moving direction of the glass slide is exactly opposite to the moving direction of the object image seen in the field of view. If the object image is not very clear, you can adjust the micro-focusing handwheel until the object image is clear.
7. In general microscopes with normal functions, the low magnification objective lens and the high magnification objective lens are basically parfocal. When the low magnification objective lens is used to observe clearly, the object image should be seen by changing the high magnification objective lens, but the object image may not be very clear, and it can be rotated and moved slightly. Adjust the focus handwheel.
8. After converting the high-power objective lens and seeing the object image clearly, you can adjust the size of the aperture diaphragm or the height of the condenser as required to make the light meet the requirements (generally, when replacing the low-power objective lens with a high-power objective lens for observation, the field of view should be slightly smaller. It gets darker, so you need to adjust the light intensity).
9. After the observation, the objective lens should be moved away from the clear hole first, then the aperture diaphragm should be adjusted to the maximum, and then the stage should be lowered slowly, and the parts should be checked for damage (especially to check whether the objective lens is stained or not). Water and oil, such as water or oil, use lens paper to wipe it off), after inspection and processing, it can be packed.
