Introduction to the Working Principle of Laser Confocal Microscope
Laser confocal microscope is a new generation of powerful research tools in the fields of morphology, molecular biology, neuroscience, pharmacology, genetics, etc., which adds a laser scanning device to fluorescence microscopy imaging and uses computer image processing to improve the resolution of optical imaging by 30% -40%. Fluorescence probes are excited by ultraviolet or visible light to obtain fluorescent images of the internal microstructure of cells or tissues. Physiological signals such as Ca2+, pH value, membrane potential, and changes in cell morphology can be observed at the subcellular level. The laser confocal imaging system can be used to observe various stained, unstained, and fluorescently labeled tissues and cells, observe and study tissue sections, the growth and development characteristics of living cells, and study and measure intracellular material transport and energy conversion. Capable of conducting studies on ion and pH changes in living cells (RATIO), neurotransmitter research, differential interference and fluorescence tomography, multiple fluorescence tomography and overlap, fluorescence spectroscopy analysis, quantitative analysis of fluorescence indicators, time delay scanning of fluorescent samples and dynamic components of three-dimensional dynamic structural components of tissues and cells, analysis of fluorescence resonance energy transfer, fluorescence in situ hybridization research (FISH), cytoskeleton research, gene localization research, in situ real-time PCR product analysis, fluorescence bleaching recovery research (FRAP), intercellular communication research, protein-protein research, membrane potential and membrane fluidity research, and completing image analysis and three-dimensional reconstruction analysis.
Application fields of laser confocal microscopy system:
Involving medicine, animal and plant research, biochemistry, biology, cell biology, tissue and embryo, food science, genetics, pharmacology, physiology, optics, pathology, botany, neuroscience, marine biology, materials science, electronics science, mechanics, petroleum geology, and mineral science.
