Inverted Microscope Why "Inverted" Microscope?
The composition of an inverted microscope is the same as that of an ordinary microscope, except that the objective lens and the illumination system are reversed, the object is located in front of the objective lens, and the distance from the objective lens is greater than the focal length of the objective lens, but less than twice the focal length of the objective lens. After passing through the objective lens, an inverted magnified real image is formed. What our eyes see through the eyepiece is not the object itself, but the magnified image of the object formed by the objective lens.
Since the materials observed by an inverted microscope are generally cultured cells, which have high transparency and inconspicuous structural contrast, an inverted microscope is often equipped with a phase-contrast objective lens, which actually constitutes an inverted phase-contrast microscope.
On an inverted microscope, various types of consumables such as Petri dishes and multi-well plates are often used. The thickness of the bottom is different, which will cause certain changes in the passage of light. At this time, it is necessary to use an objective lens with a correction ring function, which is equipped with a ring-mounted adjustment ring in the middle. When the adjustment ring is turned, the distance between the lens groups in the objective lens can be adjusted, thereby correcting the correction caused by the cover glass (petri dish). ) Aberrations caused by non-standard thickness (1.2mm for conventional petri dish, 0.17mm for cover glass). The correct way to use it is: adjust the correction ring to the standard value of 1.2mm, and focus on the sample. Adjust the correction ring to the right by half a grid, and then focus on the sample. If the image effect becomes better, then adjust to the right and then focus, otherwise adjust to the left.
Inverted Biological Microscope Realizes Dual-Channel Function The new 1 infinity optical path of the product allows you to introduce an additional light source to implement techniques such as FRAP, photoactivation, laser ablation, laser tweezers or optogenetics.
The inverted microscope was born to adapt to the microscopic observation of tissue culture, cell culture in vitro, plankton, environmental protection, food inspection, etc. in the fields of biology and medicine. Due to the special limitations of these samples, the objects to be inspected are all placed in the petri dish (or culture bottle), which requires the objective lens and condenser lens of the inverted microscope to have a long working distance, so that the objects under inspection in the petri dish can be directly microscopically Observe and study. Therefore, the positions of the objective lens, condenser lens and light source are all reversed, hence the name "inversion".
