Method for choosing a microscope objective lens
Microscopes provide customers with a variety of objective lenses with various functions. Here is a brief introduction to how to choose objective lenses according to different types of microscopes.
Objective lenses can be divided into three reference types: PLN (plan apochromat), UPLFLN (universal semi-apochromat) and UPLSAPO (universal apochromat), and there are also special corresponding phase contrast (PLN-PH, UPLFLN-PH) and corresponding polarization ( PLN-P, UPLFLN-P) objective lens selection, and corresponding to the inverted microscope observation, there are corresponding long working distance objective lenses (LUCPLFLN, CPLFLN) and so on.
The basic parameters describing the objective lens include magnification, numerical aperture (N/A), working distance (WD) and field number (F.N.). The benchmark parameters of different types of objective lenses with the same magnification will also be different.
Generally speaking, the observation methods of optical microscopes are as follows:
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BF: bright field, DF: dark field, PH: phase contrast, PO: polarized light, DIC: differential interference, FL: fluorescence observation (blue, green, purple, etc.), UVFL (ultraviolet fluorescence observation). Different objective lens types are suitable for some of these observation methods. For example, PLN (F.N.=22) type objective lens can be observed in the following ways:
In addition to the objective lens itself, the reasonable combination of the microscope objective lens and the overall optical system of different types of microscopes can achieve the best observation effect and the best price-performance ratio.
