New developments in optical microscopy techniques and the outstanding advantages of each
Fluorescence microscopy is a powerful tool for qualitative localization research on specific intracellular proteins, nucleic acids, carbohydrates, lipids, and certain ions at the light microscope level.
Laser scanning confocal microscope, with clear imaging and high resolution, is more and more widely used in studying the localization and dynamic changes of subcellular structures and components, fluorescence resonance energy transfer technology, fluorescence bleaching recovery technology and single-molecule imaging technology And so on are inseparable from the laser scanning confocal microscope.
Phase contrast microscope can observe the dynamics of living cells and organelles such as nuclei and mitochondria without staining.
Differential interference microscope, developed on the basis of phase contrast microscope, increases the difference between light and dark in the density of the sample, increases the contrast, and the imaging is more three-dimensional, which is more suitable for the study of living cells.
Dark field microscope, using scattered light to observe cells in a dark background, the edges of cells and organelles are more clear.
Inverted microscope, the illumination system and the objective lens are reversed, which increases the distance between the light collector and the stage, and can be placed in a petri dish for observation.
Video-contrast microscope, with an order of magnitude higher resolution than ordinary optical microscopes, can study living cells at high resolution and observe the movement of particles.
