Operation steps and maintenance methods of optical microscope
1. Place
Hold the mirror arm with your right hand and the mirror holder with your left hand to keep the mirror body upright. The tabletop should be clean and stable, and choose a place near a window or well-lit. The single tube is generally placed on the left side, 3 to 4 cm from the edge of the table.
2. Cleaning
Check whether the microscope is faulty and clean. The mechanical part of the mirror body can be wiped with a clean soft cloth. The lens should be wiped with lens tissue. If there is glue or dirt, it can be cleaned with a small amount of xylene.
3, the light
The lens barrel is raised to a distance of 1-2 cm from the stage, and the low magnification lens is aligned with the light aperture. Adjust the aperture and mirror, use a flat mirror when the light is strong, use a concave mirror when the light is weak, and turn the mirror with both hands.
If you are using a microscope with a light source, you can omit this step, but you will need a knob to adjust the brightness.
4. Install the specimen
Place the slide on the stage, making sure that the side with the coverslip is facing up. Secure the slide with spring clips, and turn the knob of the stage mover to align the material to be observed in the center of the light-through hole.
5. Focus
When focusing, first rotate the coarse focusing knob to lower the lens barrel slowly, and observe carefully from the side until the objective lens is close to the specimen on the slide, then observe through the eyepiece with the left eye, and turn the coarse focusing knob with the left hand to raise the lens barrel until the mark is clearly seen. Stop when the object is imaged, and then use the fine focus knob to recall the clarity.
Operation Note: The focus should not be adjusted directly under the high magnification lens; when the lens barrel is lowered, the distance between the lens barrel and the specimen should be observed from the side; the critical value of the object distance should be known.
If the binocular microscope is used, if the observer's binocular diopter is different, it can be adjusted by the diopter adjustment circle. In addition, the binoculars can be translated relative to each other to accommodate the distance between the operator's eyes.
6. Observation
If using a monocular microscope, open both eyes naturally, observe the specimen with the left eye, observe recording and drawing with the right eye, and adjust the focal length with the left hand to make the object clear and move the specimen field of view. Right hand recording and drawing.
During the microscopic examination, the specimen should be moved in a certain direction until the entire specimen is observed, so as not to miss the inspection and not to repeat it.
Adjustment of light intensity: under normal circumstances, the light for dyed specimens should be strong, and the light for colorless or unstained specimens should be weak; the light for low-power observation should be weak, and the light for high-power observation should be strong. In addition to adjusting the mirror or light source lamp, the adjustment of the iridescent aperture is also very important.
(1) Low magnification observation
When observing any specimen, low magnification must be used first because of its large field of view, it is easy to find the target and determine the part to be observed.
(2) High magnification observation
When turning from low magnification to high magnification, just slightly adjust the fine focus knob to make the image clear.
Do not use the coarse focus knob when using a high magnification lens, otherwise the cover glass may be crushed and the lens may be damaged.
When rotating the objective lens converter, do not use your fingers to push the objective lens directly, which will easily skew the optical axis of the objective lens and damage the converter threads due to uneven force, which will eventually cause the converter to be scrapped.
(3) Observation of oil mirror
First use the low-power mirror and high-power mirror to move the object to be inspected to the center of the field of view, and then change the oil mirror for observation. Before observation with oil lens, the microscope brightness should be adjusted to the brightest, and the aperture should be fully opened.
When using the oil lens, first put a drop of cedar oil (mirror oil) on the cover glass, then lower the lens barrel and observe carefully from the side until the oil lens is immersed in the cedar oil and close to the slide specimen, then observe with the eyepiece and fine tune with The focus knob lifts the lens barrel until the focal length of the specimen is clearly seen and stops and adjusts clearly.
Add cedar oil dropwise to the right amount. After the oil lens is used, be sure to wipe off the cedar oil with a lens paper dipped in xylene, and then wipe off the excess xylene with a dry lens tissue.
7. End the operation
After the observation, remove the sample, twist the converter, make the V-shaped lens on both sides, the reflector should be erected, lower the lens barrel, wipe it clean, and put on the lens cover.
If you are using a microscope with a light source, you need to adjust the brightness knob to adjust the brightness to the darkest, and then turn off the power button to prevent the light source lamp from being burnt out by an instantaneous overcurrent when the machine is turned on next time.
