Phase Contrast Observation Method for Inverted Metallurgical Microscopes
The most commonly used observation method in inverted microscopes is phase contrast. Since this method does not require staining, it is an ideal way to observe live cells and microorganisms.
The operation steps are as follows:
1. Turn on the device and connect it to the power supply. Open the electronic control switch at the bottom of the mirror body.
2. Place the object to be observed on the stage. Rotate the three hole converter and choose a smaller objective lens. Observe and adjust the hinged binocular eyepiece for comfort.
3. Adjust the light source: Push and pull the brightness adjuster at the lower end of the mirror body to the appropriate level. Adjust the size of the light source by adjusting the grating under the spotlight.
4. Adjust image distance: Use a three hole converter to select the appropriate magnification objective lens; Replace and select a suitable eyepiece; Simultaneously adjusting the lifting and lowering to eliminate or reduce the halo around the image, improving the contrast of the image.
5. Observation: Observe the results through the eyepiece; Adjust the stage and select the observation field of view.
6. Turn off the device, remove the observed object, and adjust the brightness of the light source to * dark. Turn off the switch at the bottom of the mirror and disconnect the power supply. Rotate the three hole converter to place the objective lens on the lower side of the stage to prevent dust from settling.
Definition of Inverted Metallographic Microscope
Inverted metallographic microscope is an important instrument for studying metallurgy, mainly used for identifying and analyzing the internal structure and structure of metals. Inverted metallographic microscope can be used for quality research in casting, smelting, heat treatment, inspection of raw materials, or analysis after material treatment.
The inverted metallographic microscope system is an organic combination of traditional optical microscopes and computers (digital cameras) through photoelectric conversion. It can not only perform microscopic observations on the eyepiece, but also observe real-time dynamic images on the computer (digital camera) display screen, and edit, save, and print the required images.
When operating an inverted metallographic microscope, the observation surface of the sample is downward and coincides with the worktable, and there is no requirement for the height and parallelism of the sample, making it suitable for irregularly shaped or larger samples. Inverted metallographic microscopes are widely used in factories, laboratories, teaching, and scientific research fields.
