Practical operation of debugging methods for using microscopes
1. Adjustment of microscope illumination light path system
In order to provide uniform and sufficient illumination for the field of view of the microscope, it is necessary to adjust the lighting path system during the initial installation and debugging of the microscope. This is an important means and basic requirement for correctly using the microscope and obtaining correct and reproducible results. In addition, mastering the adjustment of the lighting path system correctly is a necessary step after replacing the light source bulb during the use of the microscope, and it is also a necessary means to periodically test the performance of the microscope during daily use. The adjustment of the microscope lighting path system mainly includes the following four aspects:
(1) Preliminary adjustment of the lighting source chamber outside the microscope
① Firstly, open the outer shell of the lamp compartment and press the spring clip to insert the halogen bulb into the socket. During installation, avoid direct contact with the bulb with your fingers (use a soft cloth or paper to block it), so as not to leave fingerprints or other dirt on the bulb, which may affect its service life.
② Place the lamp room on the desktop, turn on the power, and use a dedicated screwdriver to adjust the focusing knob hole of the lamp (marked with "←→"), so that the filament is projected onto a wall 1-2m away, and the image of the filament is adjusted to be clear; Then adjust the high and low position of the lamp to adjust the filament hole (marked with "-") so that the filament position is appropriate; Adjust the left and right positions of the lamp by adjusting the screw holes (marked with "--") to ensure that the left and right positions of the filament are appropriate.
(2) The purpose of checking and correcting the position of the light-emitting body (filament) in the microscope is to align the image end of the light-emitting body into the field of view of the objective lens, ensuring sufficient and uniform illumination of the microscope's field of view from the perspective of the light source. This is a prerequisite for adjusting the Kuhler lighting system. Basic tools required: The centering telescope was equipped with a microscope when purchased.
① Remove the ground glass sleeve from the lamp compartment and install the lamp compartment back onto the microscope;
② Select a 10 x objective lens, turn on the light source program to find the sample and focus it clearly, then use a 40 x objective lens to focus the sample clearly (the 40 x objective lens can clearly see the full picture of the filament);
③ Open both the aperture and field of view apertures of the condenser lens to a larger size;
④ Remove one of the eyepieces, replace it with a centering telescope, grab the white part, and use the other hand to retract the black eyepiece to see the filament image in the field of view;
⑤ If the position of the filament is not appropriate, adjust the "--" hole and adjust the filament image horizontally. Adjust the "--" hole and adjust the filament image vertically until the filament image is adjusted to a light circle image that exactly fills the aperture of the objective lens;
⑥ After adjustment, insert the ground glass sleeve back into its original position, remove the centering telescope, and replace it with the eyepiece for the next adjustment. The adjustment of the lighting source chamber outside the microscope and the verification of the position of the light emitting body inside the microscope mentioned above only need to be carried out during the initial installation, debugging, and replacement of the light bulb of the microscope. During normal use of the microscope, it should not be adjusted or moved randomly. In case of confusion, you can follow the above steps to restore it to its original state.
