Adjustment of Microscope Illumination Light Path System
① Select 10 x objective and 10 x eyepiece;
② Place the front lens of the spotlight into the optical path, adjust the aperture aperture aperture to a moderate position (not large or small), then lift the spotlight to the top position, and adjust the spotlight dial to the bright field "J" position;
③ Set the field of view aperture to small (0.1);
④ Place sealed biological samples on the stage, turn on the light source, and focus clearly;
⑤ A locally illuminated area or bright spot will appear in the field of view, which is a blurry image of the field of view aperture where the details of the sample can be clearly seen; Outside it is a darker field of view, and it may not be possible to see the details of the sample clearly;
⑥ Lowering the spotlight slightly reduces the bright spots in the field of view, gradually forming a clear polygonal image, which is the clear image of the field of view aperture;
⑦ In general, the polygonal image is not in the center of the field of view, and it is necessary to adjust a pair of centering screws of the spotlight to bring the image of the polygonal field of view aperture to the center position;
⑧ Gradually widen the field of view aperture, making the polygonal image an inscribed polygon in the field of view, and further check the alignment status. If the alignment is not ideal, continue to slightly adjust the alignment screw;
⑨ By slightly increasing the aperture of the field of view, its polygonal image disappears precisely at the edge of the field of view. At this point, the Kuhler lighting system has been adjusted. After adjusting the Kuhler lighting system, the entire field of view is illuminated uniformly, and the microscopic photos taken are bright and clear with normal contrast. In future use, special attention should be paid to: a. The field of view aperture cannot be arbitrarily enlarged, but it can be reduced as the objective lens magnification increases, and enlarged as the objective lens magnification decreases; b. The high and low positions of the spotlight should not be adjusted randomly, otherwise it will damage the already adjusted Kuhler lighting system; c. When using an objective of less than 10 x, the front lens of the condenser should be placed out of the optical path. When using an objective of 10 x or more, the front lens should be placed into the optical path; d. Regarding the issue of matching the magnification of the objective lens with the size of the field of view aperture, in practical use, as a general observation, it is not necessary to reduce or increase the field of view aperture. However, in micrographs, in order to avoid the interference of stray light on the photography system and to capture more complete photos, it is necessary to adjust the field of view aperture to just disappear at the edge of the observed field of view when using each magnification of the objective lens. This is a relatively complex task, but it must be done. A relatively simple method is to adjust the field of view apertures corresponding to each magnification objective in advance and mark them, and then adjust them directly to the corresponding positions according to the marks when using them in the future.
