Principle of Microscope Imaging and Its Optical Path Diagram
The microscope uses the magnified imaging principle of the convex lens to magnify the tiny objects that cannot be distinguished by the human eye to the size that the human eye can distinguish. ), using the angular magnification M to represent their magnification power.
Because the opening angle of the same object to the eyes is related to the distance of the object from the eyes, it is generally stipulated that the magnification rate of the image at a distance of 25 cm from the eyes (photopic distance) is the magnification rate of the instrument. When a microscope observes an object, the angle of view is usually very small, so the ratio of the angle of view can be replaced by the ratio of its tangent.