Principles and operating steps of optical microscope imaging
During the experiment, the microscope should be placed slightly to the left on the table in front of the seat, and the mirror seat should be about 6-7cm away from the edge of the table.
2. Turn on the light source switch and adjust the light intensity to the appropriate size.
3. Rotate the objective converter so that the low magnification lens is facing the through hole on the stage. First, adjust the lens to a distance of about 1-2cm from the stage, then focus on the eyepiece with the left eye, and then adjust the height of the condenser to the maximum aperture diaphragm, so that the light enters the lens tube through the condenser, and the line of sight is bright.
4. Place the glass slide to be inspected on the stage, so that the inspected part of the slide is located at the center of the light hole, and then clamp the slide with a specimen clamp.
5. First, observe with a low magnification lens (objective lens 10X, eyepiece 10x). Before inspection, rotate the coarse focusing handwheel to raise the stage and gradually approach the glass slide with the objective lens. Attention should be paid to not involving the objective lens with the glass slide, in order to prevent the lens from crushing the glass slide. Then, focus your left eye into the eyepiece while keeping your right eye open (cultivate the habit of using a microscope to observe with both eyes open, so that you can use your right eye to look at the drawing while observing), and rotate the coarse focus handwheel to gradually lower the stage. Soon, you can see the magnified image of the material in the glass slide.
If the object seen in the line of sight does not meet the test requirements (the object deviates from the line of sight), the moving handle of the stage can be gradually adjusted. When adjusting, attention should be paid to the direction of the slide's movement being exactly opposite to the direction of the object's movement seen in the line of sight. If the object is not very clear, you can adjust the micro focus handwheel until the object is clear.
7. If a high-power objective is further used for inspection, the part of the object that needs to be magnified for inspection should be moved to the center of the line of sight before switching to a high-power objective (when switching from a low-power objective to a high-power objective for inspection, the size of the object in the line of sight is significantly reduced). Generally, microscopes with normal functions have low and high magnification objectives that are aligned with each other. When using a low magnification objective to observe clearly, replacing it with a high magnification objective should be able to see the object image, but the object image may not be very clear. It can be adjusted by turning the micro focusing handwheel.
After switching to a high-power objective and seeing the object clearly, it is possible to adjust the size of the aperture aperture or the height of the condenser according to the requirements to ensure that the light meets the requirements (generally, when replacing a low-power objective with a high-power objective for inspection, the line of sight needs to be slightly darker, so the intensity of the light needs to be adjusted).
