Six major steps of an optical microscope:
1. Microscope retrieval: ① Hold the mirror arm with the right hand; ② Left hand mirror holder; ③ Placed in front of the chest.
2. Rotation of the microscope: ① The barrel is facing forward and the arm is facing back; ② Placed on the table in front of the observer's seat, leaning towards the left side of the body, making it easier for the left eye to observe through the eyepiece; ③ Place on the inside of the table, about 5cm away from the edge of the table.
3. Aiming: ① Rotate the coarse focusing screw to slowly raise the lens tube, then rotate the converter to align the low magnification objective with the through hole; ② Rotate the sunshade (or sheet aperture) with your fingers to align the maximum aperture with the through-hole, gaze into the eyepiece with your left eye, and rotate the reflector to face the light source, making the brightness in the field of view uniform and appropriate.
4. Use of low magnification objective: ① Rotate the coarse focus screw by hand to slowly lower the lens barrel, while keeping both eyes on the objective lens from the side. Stop when the objective lens is 2-3mm away from the slide on the stage. ② Look into the eyepiece with your left eye (note that your right eye should be open at the same time), and rotate the coarse focus screw to slowly raise the lens until you can see the object clearly. If unclear, adjust the fine focus screw until clear.
5. Use of high-power objective: Before using a high-power objective, you must first use a low-power objective to find the observed object, adjust it to the center of the field of view, and then rotate the converter to replace the high-power objective. After the high-power mirror is replaced, the brightness in the field of vision will become dark. Therefore, generally select a larger aperture and use the concave surface of the reflector, and then adjust the fine focusing spiral. The number of objects viewed decreases, but the volume increases.
6. Use of Reflectors: Reflectors are usually used in conjunction with light shields (or apertures) to adjust the brightness within the field of view. Reflectors have flat and concave surfaces. When facing light, if the field of view light is too strong, use the flat surface of the reflector. If the light is still too strong, use a smaller aperture at the same time; On the contrary, if the light in the field of view is weak, use a larger aperture or use a concave mirror.
