The advantages of laser confocal microscopy in the biological field
Compared with traditional optical microscopes, laser confocal microscopy has higher resolution, multiple fluorescence, and the ability to observe and form clear three-dimensional images. In the observation of biological samples, laser confocal microscopy has the following advantages:
1. Continuous scanning of live cell tissue or cell slices can obtain detailed three-dimensional images of the cytoskeleton, chromosomes, organelles, and cell membrane system.
2. It can obtain images with higher contrast and resolution than ordinary fluorescence microscopes, while also having high sensitivity and excellent sample protection.
3. Obtaining multidimensional images. Such as 7-dimensional image (XYZa λ It): xyt, xzt, and xt scans, time series scanning, rotation scanning, region scanning, spectral scanning, and convenient image processing.
4. Intracellular ion fluorescence labeling. Single or multiple markers are used to detect the ratio and dynamic changes of intracellular concentrations such as pH and sodium, calcium, and magnesium ions.
5. Fluorescent labeling. Observation of live cell biomass, membrane markers, substances, reactions, receptors or ligands, nucleic acids, etc. in probe labeled live cells or sliced specimens; Multiple substance labeling can be performed simultaneously on the same sample and observed simultaneously.
6. Non damaging, safe, reliable, and excellent repeatability in cell detection; Data images can be output in a timely manner or stored for a long time.
