Use of a stereo microscope and precautions to take
A stereo microscope is a compact, macroscopically magnified microscope that can be used for observation, photography, analysis, and other purposes. There are also good research-grade stereo microscopes available with superior objective lenses and higher magnification, which typically magnify objects by a factor of 6–200. Since they are not very accurate for qualitative measurements, stereo microscopes are typically exclusively employed for stereotyped analysis. To finish it, you must collaborate with software or an eyepiece measurement ruler and be aware of the body's precise zoom ratio.
The following are the precise procedures for utilizing the Leica microscope:
(1) After mounting the stereo microscope, make sure the power supply voltage is in line with the stereo microscope's recommended voltage before plugging in the power plug, turning on the power switch, and choosing the illumination mode;
(2) Choose the platen, insert it into the base platen's hole, and lock it depending on the specimens to be studied (for opaque specimens, use a black-and-white platen; for transparent specimens, use a frosted glass platen);
(3) Loosen the focusing slider's fastening screw, then raise or lower the mirror body until it is roughly within working distance with the chosen objective lens's magnification. Lock the focusing bracket after adjusting it, then lock the safety ring in place;
(4) Insert the eyepiece; before tightening the screw on the eyepiece tube, loosen it first. When inserting the eyepiece into the stereo microscope eyepiece tube, take care not to touch the lens' surface.
(5)Interpupillary distance should be adjusted. The two prism boxes should be changed in order to alter the exit pupil distance of the eyepiece tube so that the user can observe a circular field of view once they have done so through the two eyepieces. a circle whose range of vision completely overlaps (this signifies that the interpupillary distance has been corrected);
(6) Observe the specimen (focus on the specimen). First adjust the dioptric ring on the left eyepiece tube to the position of the 0 reticle. Normally, first observe from the right eyepiece tube (i.e. fixed eyepiece tube), turn the zoom tube (for models with a zoom device) to the highest position, turn the focusing handwheel to focus on the specimen until the specimen’s After the image is clear, turn the zoom tube to the lowest magnification position. At this time, observe with the left eyepiece tube. If it is not clear, adjust the diopter ring on the eyepiece tube along the axial direction until the image of the specimen is clear. Visually observe its focusing effect;
(7) After the observation is over, shut off the power, take the sample out of the microscope, and tightly cover it with a dust cover.
Leica microscope safety measures:
(1) The instrument should be protected from direct sunshine, extreme heat, humidity, dust, and acid and alkali gas corrosion;
(2) After use, the stereo microscope should be covered with a dust cover in the workshop to maintain cleanliness;
(3) A solid desk should be used to position the stereo microscope;
(4) When using the stereo microscope, take care to keep your hands clean to prevent dirt from getting on the lens and color filter.
