Use of microscope oil immersion objective
When using oil-immersion objectives, you should generally not use same-height focusing. Same-height focusing applies only to the original objectives of each microscope, and is an extremely favourable convenience when using low and high magnification objectives, but is somewhat limited when using oil-immersion objectives. Generally speaking, when observing specimen slices (slides) without coverslips with an oil microscope, the accuracy of focusing using same-height focusing is greater, and it should be used with caution for specimen slices with coverslips, as the working distance of an oil-immersion objective lens is very The concentric height considered in the design and assembly is for standard thickness coverslips.
When using an oil immersion objective, apply only a drop of cedar oil to the specimen sheet. After observation, cleaning work should be carried out in time, if not carried out in time, the cedar oil sticks to dust, dust particles may wear the lens when wiping, the cedar oil in the air exposed for a long time, it will also thicken and dry, wiping is very difficult, and is very unfavourable to the instrument. Wiping should be done carefully and gently. The front end of the oil-immersed objective lens is first wiped once or twice with dry wiping paper to remove most of the oil, then wiped twice with xylene-dripping wet wiping paper, and finally wiped once again with dry wiping paper. Specimen piece of cedar oil can be used to 'pull the paper method' (that is, a small piece of microscope paper covered in cedar oil, and then drop some xylene on the paper, while wet to pull the paper outward, so that three or four times in a row, can be clean, generally will not be damaged by the smear specimens without coverslips) to wipe clean. Mirror paper should also be dustproof, generally before use, each page will be cut into 8 small pieces, stored in a small clean petri dish, with both savings and convenience.
