What should you pay attention to when using an oil lens to observe with a microscope?
Before using the oil lens, you must first observe through low and high magnification lenses, and then move the part that needs further magnification to the center of the field of view.
Raise the light collector to its highest position and open the aperture to its maximum.
Turn the converter to move the high-power lens away from the light aperture, add a drop of cedar oil on the glass slide of the area to be observed, and then slowly turn the oil lens. When changing the oil lens, watch the distance between the lens and the glass plate horizontally from the side. It is better to immerse the lens in oil without crushing the glass slide.
Observe the eyepiece with your left eye and slowly turn the fine adjuster until the object image is clear.
If there is no object image or the target is not ideal and it is necessary to search again, follow the procedure of low magnification → high magnification → oil lens when searching again outside the refueling area. When re-searching in the refueling area, follow the procedure: low magnification → oil lens. High magnification lens is not allowed to avoid oil contamination of the lens.
After using the oil lens, first wipe it with lens paper, then use lens paper dipped in a little xylene to wipe off the cedar oil on the lens and specimen, and finally wipe it clean with dry lens paper.
Why does an optical microscope have the highest resolution when using an oil lens?
Oil lens is one of the optical microscopes. When used, the lens is immersed in oil (usually cedar oil) to observe finer structures. It is one of the microscopes commonly used in laboratories. The clarity is slightly higher than that of ordinary optical microscopes. It is used for Observe chlamydia, bacteria, cell organelles, etc.
The lens of the oil lens is very small. When the light passes through the air between the glass slide and the oil lens, refraction or total reflection occurs due to the different density of the medium, which reduces the light entering the lens and makes the object image unclear. If the oil lens and the carrier are Adding cedar oil (n=1.515), which is similar to the refractive index of glass (n=1.52), between the glass slides will increase the light entering the lens, enhance the brightness of the field of view, and make the object image bright and clear.
Due to the small size of bacteria, in morphological studies of bacteria, it is often necessary to use a microscope oil lens to observe more clearly. Therefore, one must be proficient in the use and protection of oil lenses.
