What are the differences between the use of oil lenses and ordinary objective lenses?
When using the oil lens, you need to drop a drop of cedar oil on the glass slide, and the oil drop cannot be separated from the objective lens during its ascent. Moreover, the magnification of the oil lens is larger than that of the ordinary objective lens.
When using oil lenses, you should pay attention to:
1. After using the oil lens, first use lens cleaning paper dipped in a little xylene to wipe off the cedar oil on the lens and specimen, and then wipe it clean with dry lens tissue. After adding cedar oil, the field of view becomes obviously darker. You need to raise the light collector to the highest position and open the aperture to the maximum. Before using the oil lens, the target observed through the low-power lens and the high-power lens must be in the center of the field of view.
2. Cedar oil is a special oil for oil lenses. Dropping a liquid with a refractive index of 1.5 under the 100x oil lens can significantly increase the resolution of the oil lens and improve the observation effect of the microscope. The refractive index of cedar oil is 1.52, which is the Good microscope oil lenses use oil.
3. When using a microscope oil lens, the microscope must be upright on the table. The arms must not be bent to tilt the stage to prevent the cedar oil from overflowing, affecting observation and contaminating the table.
The principle of oil lens:
The oil microscope is one of the commonly used microscopes in the laboratory. Its clarity is slightly higher than that of ordinary optical microscopes. It is used to observe finer structures such as chlamydia, bacteria, and cell organelles.
When using an oil lens, drop drops of cedar oil on the slide. This is because the magnification of the oil lens is high and the lens is very small. When light passes through media objects of different densities (glass slide → air → lens), part of the light will be refracted and lost. Less light will enter the lens barrel and the field of view will be smaller. Dark, objects cannot be seen clearly. If cedar oil (n=1.515), which is similar to the refractive index of glass (n=1.52), is dropped between the lens and the glass, the light entering the oil lens will increase, the brightness of the field of view will be enhanced, and the object image will be clear.
