Why can't electron microscopes replace light microscopes?
Electron microscopes use the principle of electron optics, replacing light beams and optical lenses with electron beams and electron lenses, so that the fine structures of substances can be imaged at very high magnifications. Although its resolving power is far better than that of optical microscopes, electron microscopes are difficult to observe living organisms because they need to work under vacuum conditions, and the irradiation of electron beams will also cause radiation damage to biological samples, so it cannot completely replace optical microscopes. Microscopes, and their costs are different, and their suitable working ranges are also different. Hope my answer helps you.
The reasons why electron microscopes cannot completely replace light microscopes are as follows:
1. The electron microscope belongs to the optical microscope and adds accessories such as CCD, display screen or computer. This can only be said to be a video microscope. During the entire imaging process, the CCD replaces the human eye. Because in video imaging, electronic magnification is virtual magnification, and in terms of pixels, photosensitive effects and other factors, it is too different from the human eye, so it is too different from the visual microscope effect;
2. Another most important reason is that CCD is a plane imaging, and the human eye, especially in the case of binocular observation, will produce a strong sense of three-dimensionality, which is the reason why the contrasting depth of field effect is too large;
3. Electron microscopes are mostly expressed as electron scanning microscopes. The effect of this microscope is much better than that of general optical microscopes, but because of its high price, it is rarely used in industry.
