Why is a microscope that is "inverted" called that?
The components of an inverted microscope are identical to those of a regular microscope, with the exception that the objective lens and illumination system are switched around, the object is placed in front of the objective lens, and the distance between the objective lens and the subject is greater than the objective lens's focal length but not twice as great. A genuine picture that has been enlarged and inverted forms after going through the objective lens. The item's magnified picture, created by the objective lens, is what our eyes see via the eyepiece rather than the actual object.
An inverted microscope is frequently equipped with a phase-contrast objective lens, which actually comprises an inverted phase-contrast microscope. This is because the materials examined by an inverted microscope are typically cultivated cells, which exhibit high transparency and subtle structural contrast.
On an inverted microscope, various types of consumables such as Petri dishes and multi-well plates are often used. The thickness of the bottom is different, which will cause certain changes in the passage of light. At this time, it is necessary to use an objective lens with a correction ring function, which is equipped with a ring-mounted adjustment ring in the middle. When the adjustment ring is turned, the distance between the lens groups in the objective lens can be adjusted, thereby correcting the correction caused by the cover glass (petri dish). ) Aberrations caused by non-standard thickness (1.2mm for conventional petri dish, 0.17mm for cover glass). The correct way to use it is: adjust the correction ring to the standard value of 1.2mm, and focus on the sample. Adjust the correction ring to the right by half a grid, and then focus on the sample. If the image effect becomes better, then adjust to the right and then focus, otherwise adjust to the left.
Inverted Biological Microscope Realizes Dual-Channel Function The new 1 infinity optical path of the product allows you to introduce an additional light source to implement techniques such as FRAP, photoactivation, laser ablation, laser tweezers or optogenetics.
The inverted microscope was born to adapt to the microscopic observation of tissue culture, cell culture in vitro, plankton, environmental protection, food inspection, etc. in the fields of biology and medicine. Due to the special limitations of these samples, the objects to be inspected are all placed in petri dishes (or culture bottles), which requires that the objective lens and condenser lens of the inverted microscope have a long working distance, and can directly microscopically examine the objects in the petri dish. Observe and study. Therefore, the positions of the objective lens, condenser lens and light source are all reversed, hence the name "inversion".
