Why Optical Microscopes When Using Oil Objectives Have the Highest Resolution
Oil lens, one of the optical microscopes, when used, the lens is immersed in oil (usually cedar oil), used to observe finer structures, is one of the microscopes commonly used in laboratories, the definition is slightly higher than ordinary optical microscopes, used to observe chlamydia, bacteria, organelles, etc.
The lens of the oil lens is very small. When the light passes through the air between the glass slide and the oil lens, refraction or total reflection occurs due to the different density of the medium, which reduces the light entering the lens and makes the object image unclear.
The light entering the lens increases, the brightness of the field of view is enhanced, and the object image is bright and clear
Due to the small size of bacteria, in the morphological study of bacteria, it is often necessary to use a microscope oil lens to observe clearly. Therefore, it is necessary to be proficient in the use and protection of the oil lens
What problems should be paid attention to when observing the microscope with an oil lens
Before using the oil lens, it must be observed through low and high magnification lenses, and then the part to be further enlarged should be moved to the center of the field of view.
Raise the collector to the highest position and open the aperture to the maximum.
Turn the converter so that the high-magnification lens is away from the light hole, drop a drop of cedar oil on the glass slide at the part to be observed, and then slowly turn the oil lens. When switching the oil lens, watch the distance between the lens and the glass slide horizontally from the side, so that the lens is immersed in the oil without crushing the glass slide.
Observe the eyepiece with the left eye, and slowly turn the fine adjuster until the object image is clear.
If there is no object image or the target is not ideal, you need to search again. When refinding outside the refueling area, you should follow the procedure: low magnification→high magnification→oil lens. When re-finding in the refueling area, you should follow the procedure: low magnification → oil lens, and do not go through the high magnification lens to prevent oil from contaminating the lens.
After using the oil lens, first wipe it with a lens cleaning paper, then wipe off the cedar oil on the lens and the specimen with a lens cleaning paper dipped in a little xylene, and finally wipe it clean with a dry lens cleaning paper.
