Difference between inverted microscope and stereo microscope
The previous talk is about the way of microscopy of the orthostatic microscope, the observation of the main section. And inverted microscope is to adapt to biology, medicine and other fields of tissue culture, cell culture, plankton, environmental protection, food inspection and other microscopic observation. However, the limitations of the characteristics of the above samples, the object to be examined are placed in the petri dish (or culture flask), which requires inverted microscope objective lens and spotting scope has a very long working distance, can be directly on the petri dish in the object to be examined for microscopic observation and research. Therefore, the position of the objective lens, condenser lens and light source are reversed, so it is called 'inverted microscope'. Due to the limitation of working distance, the objective lens of inverted microscope has a magnification of 60 X. Generally, inverted microscopes for research are equipped with 4X, 10X, 20X, and 40X phase contrast objective lenses because inverted microscopes are mostly used for colourless and transparent observation of living organisms. If users have special needs, other accessories can also be used to complete the differential interference, fluorescence and simple polarisation observation. Currently, inverted microscope is widely used in patch-clamp, transgene ICSI and other fields.
Stereo microscope (Stereo microscope) Stereo microscope, also known as 'solid microscope' or 'anatomical microscope', is a kind of three-dimensional sense of the positive image of the visual instrument, is widely used in biology, medicine, agriculture, forestry, industry and marine biology departments. It has the following characteristics: 1. binocular tube in the left and right beams are not parallel, but has a certain angle - body angle (generally 12 degrees - 15 degrees), so the image has a three-dimensional sense of three-dimensional; 2. elephant is upright, easy to operate and dissection, which is due to the prism below the eyepiece to the elephant inverted the sake of the 3. Although the magnification of the conventional microscope, but the working distance is very long! 4. large depth of focus, easy to observe the whole layer of the object to be examined. 5. large diameter of the field of view. The current optical structure of the stereomicroscope is: by a common primary objective lens, the object after the image of the two beams of light by two groups of intermediate objective lenses ---- zoom lens to separate, and into a single perspective and then by their respective eyepieces into the image, its magnification changes by changing the distance between the intermediate mirror group and obtain, so it is also known as the 'continuous zoom stereo microscope' (Zoom-stereo microscope). With the requirements of application, the Zoom-stereo microscope can be equipped with a wealth of optional accessories, such as fluorescence, photography, photography, cold light source and so on.
