Instructions for using microscopes
(1) During the experiment, the microscope should be placed on the desktop, and the mirror holder should be about 6-7cm away from the edge of the table. Turn on the bottom light source switch.
(2) Rotate the converter so that the low magnification lens is facing the through hole on the stage. Then gaze into the eyepiece with both eyes, adjust the intensity of the light source, raise the spotlight, set the iris aperture to maximum, and reflect the light into the mirror. At this point, the field of view appears bright.
(3) Place the observed piece on the stage so that the observed part is located at the center of the through-hole.
(4) First, observe with a low magnification lens (objective lens 10 x, eyepiece 10 x). Before observation, rotate the coarse focus screw to raise the stage and gradually bring the objective lens closer to the slice. Please note that the objective lens should not touch the glass slide to prevent it from being crushed by the lens. And rotate the coarse focus screw to slowly lower the stage, and soon you can see the magnified image of the material in the glass slide.
(5) If the object seen in the field of view does not meet the experimental requirements (the object deviates from the field of view), the ruler can be slowly moved left and right. When moving, it is important to note that the direction of the slide's movement is exactly opposite to the direction of the object's movement in the field of view. If the object image is not very clear, you can adjust the fine focus spiral until the object image is clear.
(6) If using a high-power objective for further observation, the part of the object that needs to be magnified for observation should be moved to the center of the field of view before switching to a high-power objective (when switching from a low-power objective to a high-power objective for observation, the range of the object in the field of view is much reduced). A microscope with normal functions usually has a low magnification objective and a high magnification objective that are basically in focus. When observing clearly with a low magnification objective, replacing the high magnification objective should show the object image, but the object image may not be very clear. The fine focus spiral can be rotated for adjustment.
(7) After switching to a high-power objective and seeing the object clearly, the aperture or spotlight can be adjusted as needed to meet the requirements of the light (generally, when switching from a low-power objective to a high-power objective for observation, the field of view needs to be slightly darker, so the intensity of the light needs to be adjusted).
(8) After observation, the objective lens should be moved away from the light hole first, and then the microscope should be restored. And check for any damage to the parts (especially pay attention to whether the objective lens is wet, if it is wet, wipe it clean with lens paper). After inspection and handling, it can be put back in its original place.
