The alignment steps of a microscope
1. Adjust the aperture. Choose the appropriate aperture (use a small aperture for good lighting, use a high aperture for poor lighting, and switch to a larger aperture in sequence). A bright white field of view can be seen from the eyepiece.
2. Place the loading onto the stage, generally ensuring that the tissue is observed in the center of the light hole. Only in this way can imaging be achieved.
3. Adjust the coarse adjustment knob to determine the position of the tissue to be observed (initially lower the lens to be close to the mounting but not in contact, gradually adjust upwards). Simultaneously move the loading left and right, searching for the part you want to see (move left if you want to see the left side, move down if you want to see the bottom, it's the same as looking directly).
4. Replace the high-power lens for observation, adjust the fine adjustment knob (similar to the coarse adjustment knob, but more fine), so that the cell morphology to be observed can be clearly seen.
be careful:
① The lens needs to be changed from a low magnification lens because the higher the magnification, the smaller the field of view.
② When adjusting both focus knobs, they should be adjusted slowly so as not to cause damage to the microscope and not to miss the layer of cells you are looking for.
③ When lowering the lens, do not press against the cover glass, as this can easily damage the mount and further damage the lens.
Five types of incorrect usage instructions for microscopes:
1. Place the microscope next to the sink or window in the laboratory. Moisture and dust are the killers of the microscope. Improper placement and placement can greatly shorten the lifespan of the microscope.
2. Beginners of microscopes have little understanding of their basic principles and structures, and rough workmanship can also be a fatal blow to the vulnerability of microscopes. For example, frequent movement, excessive force during handling, forceful lifting and lowering of the focusing system during focusing, inability to adjust the focus and pupil distance correctly, and so on.
3. The working distance of high-power lenses is usually very short, and improper operation can also damage the high-power lens. For example, it is a bad habit for operators to not clean or wipe the 100X oil lens when using it.
When converting the objective lens, operators often directly rotate the animal lens, but little do they know that this operation will affect the coaxiality and alignment of the optical axis. The correct method of use is to rotate the objective lens converter to obtain the desired observation objective.
5. Opening the spotlight or field of view light bar too large or too small, and adjusting its position too high or too low, cannot obtain microscopy images with better resolution and sharpness. In short, to obtain better microscopic images with a good microscope, it is important to understand and observe more in order to become a master of the microscopic world.
