The difference between fluorescence microscopy and laser confocal microscopy
Different principles
1. Fluorescent microscope: It uses ultraviolet light as the light source to illuminate the object being tested, causing it to emit fluorescence, and then observe the shape and location of the object under the microscope.
2. Laser confocal microscope: On the basis of fluorescence microscope imaging, a laser scanning device is installed to excite the fluorescence probe using ultraviolet or visible light.
Different characteristics
1. Fluorescence microscope: used to study the absorption, transportation, distribution, and localization of intracellular substances. Some substances in cells, such as chlorophyll, can emit fluorescence after being exposed to ultraviolet radiation; Some substances themselves may not emit fluorescence, but if stained with fluorescent dyes or fluorescent antibodies, they can also emit fluorescence under ultraviolet radiation.
2. Laser confocal microscope: Using a computer for image processing to obtain fluorescence images of the internal microstructure of cells or tissues, and to observe physiological signals such as Ca2+, pH value, membrane potential, and changes in cell morphology at the subcellular level.
Different uses
1. Fluorescence microscope: Fluorescence microscope is a fundamental tool in immunofluorescence cytochemistry. It is composed of main components such as light source, filter plate system, and optical system. It is the use of a certain wavelength of light to excite the specimen and emit fluorescence, which is magnified through an objective and eyepiece system to observe the fluorescence image of the specimen.
2. Laser confocal microscopy: Laser scanning confocal microscopy technology has been used for research on cell morphology localization, three-dimensional structural recombination, dynamic change processes, and provides practical research methods such as quantitative fluorescence measurement and quantitative image analysis. Combined with other related biotechnology, it has been widely applied in molecular cell biology fields such as morphology, physiology, immunology, genetics, etc.
