The difference between metallographic microscope and biological microscope

Feb 01, 2024

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The difference between metallographic microscope and biological microscope

 

Biological microscopes are familiar to everyone, but many people don't know what a metallographic microscope does? Today I will introduce to you the difference between metallographic microscope and biological microscope. The first microscope that the editor came into contact with was a biological microscope. In the biology class of junior high school, I used a biological microscope to observe onion slices and cells. Biological microscopes are used to observe and study biological slices, biological cells, bacteria, living tissue culture, fluid sedimentation, etc. It can also observe other transparent or translucent objects as well as powders, fine particles and other objects. The metallographic microscope is an optical microscope used to observe the internal microstructure of metals or non-metals.


The main difference between a metallographic microscope and a biological microscope is that the metal sample is opaque and requires light to illuminate the sample surface. Unlike biological microscopy, metallographic microscopy must use reflected light rather than transmitted light.


The following figure briefly introduces the optical path diagram of a metallographic microscope. The prepared metallographic sample is placed on the stage. The surface of the sample is perpendicular to the optical axis of the microscope. The light from the surface of the bulb or arc lamp is perpendicular to the optical axis of the microscope. The light is emitted from the bulb or arc lamp and shines through the objective lens. On the surface of the specimen, the light is collected behind the lens and passes through a semi-reflective prism into a beam parallel to the optical axis. Then it reaches the specimen surface through the objective lens, and then the light is reflected by the objective lens on the specimen surface, then enters the semi-reflective prism, and finally reaches the eyepiece to the observer's eyes and camera.

 

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