The key knowledge points of a three-dimensional ultra depth of field microscope are easy to learn
Due to the very small working distance of a 3D ultra depth of field microscope, everyone must be careful when using an oil lens to prevent damage to the lens and sample slide on the objective lens during operation.
The process of use usually follows the process of starting from a low-power mirror, then going to a high-power mirror, and then going to an oil mirror. If you are using a high-power lens, then there is no need to switch to a low-power lens and start over, just switch directly to an oil lens.
If the microscope used has a descent arrest function, then when using it, add one drop of cedar oil onto the observed slide, move the oil mirror down into the oil droplet until the descent stops, and then use fine adjustments to make fine adjustments until a clear image is obtained; If the microscope used does not have an automatic stop function, then after adding tar to the slide, when moving the objective down, it should be viewed from the side. Move the objective down to slightly contact the slide, and then perform fine adjustments upwards until the focus is aligned.
The working principle of a three-dimensional ultra depth of field microscope:
1. Refraction and refractive index:
Light propagates in a straight line between two points in a uniform isotropic medium. When passing through transparent objects of different densities, refraction occurs, which is caused by the different propagation speeds of light in different media. When light rays that are not perpendicular to the surface of a transparent object (such as glass) are emitted by air, the direction of the light rays changes at its interface and forms a refraction angle with the normal.
2. Lens performance:
Lenses are the basic optical components that make up the optical system of a three-dimensional ultra depth of field microscope. The objective, eyepiece, and condenser components are all composed of a single or multiple lenses. According to their different shapes, they can be divided into two categories: convex lenses (positive lenses) and concave lenses (negative lenses).
When a beam of light parallel to the optical axis intersects at a point after passing through a convex lens, this point is called the "focal point", and the plane passing through the intersection and perpendicular to the optical axis is called the "focal plane". There are two focal points, the focal point in the object space is called the "object focal point", and the focal plane at this point is called the "object focal plane"; On the contrary, the focal point in the image space is called the "image focal point", and the focal plane at that point is called the "image focal plane".
After passing through a concave lens, light forms an upright virtual image, while a convex lens forms an upright real image. Real images can be displayed on the screen, while virtual images cannot.
